… Flavohemoglobin dioxygenase function as studied in the present work, relies on ligand (oxygen
and nitric oxide) migration to the active site (left panel), chemical reaction between NO …

… of Escherichia coli to radical dot NO poisoning and aconitase inactivation [49], [50] led my lab
to the biochemical and genetic identification of a flavoHb … NO dioxygenase (NOD) function (…

… NOD function of E. coli flavoHb. The observations offered a distinct and common enzymatic
… analyses [394] suggest an important role for water molecules bridging the FAD and heme in …

… Previously, we demonstrated an NO • sensitivity of the Escherichia coli and the … water
molecule that are thought to act as an electron conduit (26). Spectroscopic studies of the E…

… Escherichia coli expresses an inducible flavohemoglobin possessing robust NO dioxygenase
… Dioxygen binds tightly to reduced flavohemoglobin, with association and dissociation rate …

… to water (36). In particular, reduced flavoproteins have been implicated in this redox process
in Escherichia coli (… One is a route including a dioxygenase (11, 16), where the heme-bound …

… Nitric-oxide dioxygenase (NOD) and reductase (NOR) activities of flavohemoglobin (flavoHb…
of yeast extract, and 10 g of NaCl in 1 liter of water containing 66 mm K 2 HPO 4 and 33 mm …

… activate the NO dioxygenase function of Escherichia coli flavohemoglobin. Steady-state and
… E and F-helices and switches an electron transfer gate formed by LysF7, GlnE7, and water. …

… defenses in Escherichia coli led to the isolation of an NO-inducible NAD(P)H, O 2 , and
FAD-dependent NOD 1 activity. This enzyme activity was identified with the flavoHb encoded by …

… flavoHb from Escherichia coli (HMP) has been the object of a large number of investigations
to unveil its physiological role … of NO to NO 3 − (NO-dioxygenase activity) both in vivo and in …